Molecular Characterization of Thirteen Oilseed Brassica L ...
The isolated protein sample was directly resolved with 10.0% polyacrylamide as separating gel and 4.0% as stacking gel during electrophoresis. Then, 200 μgm of each sample protein was loaded with 2X diluted Bromo Phenol Blue (BPB) loading dye (0.5 M Tris-HCl, pH 6.8; glycerol and deionised doubled distilled water in a volumetric ratio of 1: 1 ...
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