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polyacrylamide gel kit afghanistan

  • PolyAcrylamide Gel Electrophoresis (PAGE) - 1 purification

    2019-12-19u2002·u2002PolyAcrylamide Gel Electrophoresis (PAGE) - 1 purification. In PAGE Purification method, oligonucleotides are loaded on a polyacrylamide gel with the best appropriated percentage of acrylamide to purify the desired full-length oligonucleotide. The major product obtained is the slowest migrating band, which is excised out of the gel under low ...

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  • Gel Drying Kit - Promega

    Gel Drying Kit. 1 kit. Your price: The Gel Drying Kit provides a convenient and economical alternative to expensive and sometimes problematic gel dryers and vacuum systems. Both polyacrylamide and agarose gels may be dried using this kit. After electrophoresis, gels are placed between two moistened sheets of clear cellulose film, the sheets are ...

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  • Gel Drying Kit - Promega

    The Gel Drying Kit provides a convenient and economical alternative to expensive and sometimes problematic gel dryers and vacuum systems. Both polyacrylamide and agarose gels may be dried using this kit. After electrophoresis, gels are placed between two moistened sheets of clear cellulose film, the sheets are clamped between the frames, and ...

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  • Introduction to Polyacrylamide Gels

    1 day agou2002·u2002Introduction to Polyacrylamide Gels. Polyacrylamide is ideal for protein separations because it is chemically inert, electrically neutral, hydrophilic, and transparent for optical detection at wavelengths greater than 250 nm. Additionally, the matrix does not interact with the solutes and has a low affinity for common protein stains.

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  • Custom Oligo Purifications

    Polyacrylamide Gel Electrophoresis (PAGE) separates oligonucleotides variating from only 1 base and gives a purity level of 85-90%. Gel band is excised under low intensity UV. Oligonucleotide is then eluted, precipitated, quantified and packaged. Dual HPLC (double RP or RP+IEX) increases the purity level up to 95%

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  • Phos-tag™ SuperSep™ 17.5% acrylamide 17 well pre-cast ...

    Phos-tag SDS-PAGE is a phosphate affinity electrophoresis method acting as a Gel Shift Assay to distinguish between proteins in phosphorylated and non-phosphorylated states. This pre-cast gel contains 17.5% polyacrylamide and has 17 wells with a volume of 25µl.

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  • Polyacrylamide Hydrogel: An Alternative Osteoarthritis ...

    2020-3-25u2002·u2002Polyacrylamide Hydrogel: An Alternative Osteoarthritis Treatment Option. Pilot study: 82% of unsound racehorses with osteoarthritis treated …

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  • Western Blotting Protocol for Characterizing Extracellular ...

    Load the desired volume (e.g. 35 µL) of samples per well in a 10% polyacrylamide gel. Run SDS-PAGE for 30 minutes at 200 V. Rinse gel twice with distilled water. Incubate gel in cold Transfer Buffer for 15 minutes. IV. Immunoblotting and Imaging; Assemble transfer sandwich as per instrument instruction and then transfer the proteins onto a ...

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  • Genome-wide simple sequence repeats (SSR) markers ...

    2021-5-11u2002·u2002The resultant mixture was denatured for 5 min at 94 °C and then visualized on 6% denatured polyacrylamide gel using a 4300 DNA analyzer (LI-COR, Inc., Lincoln, Nebraska, USA).

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  • Antibody-Free Assay for RNA Methyltransferase Activity ...

    For urea denaturing polyacrylamide gel. Thermomixer with SMARTBLOCK 24X 1.5mL TUBES: eppendorf: 5382000023/5361000038: For temperature controlled incubation of 1.5 mL tubes. TOPO TA Cloning Kit: life technologies: Kits for fast cloning of Taq polymerase–amplified PCR products into vectors containing T7 and/or SP6 promoters for in vitro RNA ...

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  • how to use 15 cationic polyacrylamide tbe urea gel from ...

    Fractionation of soil humic acids by preparative polyacrylamide gel electrophoresis in the presence of concentrated urea. 2003). Polyacrylamide gel electrophoresis (PAGE) has been used to separate humic substances based on the differences in their molecular size (Castagnola et al. 1979; de Gonzalez et al. 1981).

    Get Price
  • Native Gel Analysis - UNC School of Medicine

    strips migrates into the gel. The leading and trailing ions (acetate/L-alanine) form a boundary that migrates through the gel leaving behind a region of uniform voltage and constant pH (pH 8.8). As this boundary passes the point of sample application (after 10 Vh) the proteins are applied to the gel. The pH in the gel is 8.8 so proteins with ...

    Get Price
  • SDS-PAGE - Assay-Protocol

    2019-7-31u2002·u2002Download SDS-PAGE protocol as a PDF . SDS-PAGE, with full name of sodium dodecyl sulfate polyacrylamide gel electrophores, is the most widely used technique to separate proteins from complicated samples of mixture, plays key roles in molecular biology and wide range of subfield of biological research. Being present a electricity, proteins migerate towards the …

    Get Price
  • Gel electrophoretic separation of proteins from cultured ...

    2014-11-5u2002·u2002Neuroendocrine tumors (NET) often develop asymptomatically and are detected at a late stage. Currently, there exist certain markers of NET that occur only in the advanced stages of the disease. Still, there is need to develop markers specific of the early stage of cancer development. Nevertheless, biomarkers are mostly low‑abundant proteins and require …

    Get Price
  • STR Analysis for Forensic and Paternity Testing

    STR analysis is useful in forensic, paternity and relationship-testing applications. Promega offers STR amplification systems to amplify CODIS loci, ENFSI-recommended loci and other loci such as the low-stutter pentanucleotide markers Penta D and Penta E.

    Get Price
  • Breast Cancer Genetics - Electrophoresis Education kit ...

    2022-1-6u2002·u2002Each kit included enough DNA, agarose gel, electrophoesis buffer and DNA stain for up to 8 groups of students (8 gels). All you need is a horizontal electrophoresis system, power supply and pipettes, all available from Cleaver Scientific. This unit has a 12 month limited warranty, for more information, consult our terms and conditions.

    Get Price
  • Denaturing Gradient Gel Electrophoresis - Cleaver Scientific

    2022-1-10u2002·u2002This technique is called denaturing gradient gel electrophoresis and is explained in more detail below. Figure 1: Melting temperature of DNA is correlated to the % content of G-C bonds in the sequence. The higher the number of GC bonds, the higher the melting temperature. Because even single base pair changes in DNA sequence can influence the T ...

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  • Frontiers

    Dictyostelid social amoebae are a highly diverse group of eukaryotic soil microbes that are valuable resources for biological research. Genetic diversity study of these organisms solely relies on molecular phylogenetics of the SSU rDNA gene, which is not ideal for large-scale genetic diversity study. Here, we designed a set of PCR–single-strand conformation polymorphism …

    Get Price
  • Gel Electrophoresis Equipment Selection Guide: Types ...

    1 day agou2002·u2002Three types of gel are used in the electrophoresis process: Agarose gels have low resolution but a high range of separation. They are employed in DNA fragment analysis. Polyacrylamide gels are used for protein and small-fragment DNA analysis. Starch gels are formed from partially hydrolyzed potato starch.

    Get Price
  • Antibody-Free Assay for RNA Methyltransferase Activity ...

    For urea denaturing polyacrylamide gel. Thermomixer with SMARTBLOCK 24X 1.5mL TUBES: eppendorf: 5382000023/5361000038: For temperature controlled incubation of 1.5 mL tubes. TOPO TA Cloning Kit: life technologies: Kits for fast cloning of Taq polymerase–amplified PCR products into vectors containing T7 and/or SP6 promoters for in vitro RNA ...

    Get Price
  • how to use 15 cationic polyacrylamide tbe urea gel from ...

    Fractionation of soil humic acids by preparative polyacrylamide gel electrophoresis in the presence of concentrated urea. 2003). Polyacrylamide gel electrophoresis (PAGE) has been used to separate humic substances based on the differences in their molecular size (Castagnola et al. 1979; de Gonzalez et al. 1981).

    Get Price
  • Native Gel Analysis - UNC School of Medicine

    strips migrates into the gel. The leading and trailing ions (acetate/L-alanine) form a boundary that migrates through the gel leaving behind a region of uniform voltage and constant pH (pH 8.8). As this boundary passes the point of sample application (after 10 Vh) the proteins are applied to the gel. The pH in the gel is 8.8 so proteins with ...

    Get Price
  • SDS-PAGE - Assay-Protocol

    2019-7-31u2002·u2002Download SDS-PAGE protocol as a PDF . SDS-PAGE, with full name of sodium dodecyl sulfate polyacrylamide gel electrophores, is the most widely used technique to separate proteins from complicated samples of mixture, plays key roles in molecular biology and wide range of subfield of biological research. Being present a electricity, proteins migerate towards the …

    Get Price
  • Gel electrophoretic separation of proteins from cultured ...

    2014-11-5u2002·u2002Neuroendocrine tumors (NET) often develop asymptomatically and are detected at a late stage. Currently, there exist certain markers of NET that occur only in the advanced stages of the disease. Still, there is need to develop markers specific of the early stage of cancer development. Nevertheless, biomarkers are mostly low‑abundant proteins and require …

    Get Price
  • STR Analysis for Forensic and Paternity Testing

    STR analysis is useful in forensic, paternity and relationship-testing applications. Promega offers STR amplification systems to amplify CODIS loci, ENFSI-recommended loci and other loci such as the low-stutter pentanucleotide markers Penta D and Penta E.

    Get Price
  • Breast Cancer Genetics - Electrophoresis Education kit ...

    2022-1-6u2002·u2002Each kit included enough DNA, agarose gel, electrophoesis buffer and DNA stain for up to 8 groups of students (8 gels). All you need is a horizontal electrophoresis system, power supply and pipettes, all available from Cleaver Scientific. This unit has a 12 month limited warranty, for more information, consult our terms and conditions.

    Get Price
  • Denaturing Gradient Gel Electrophoresis - Cleaver Scientific

    2022-1-10u2002·u2002This technique is called denaturing gradient gel electrophoresis and is explained in more detail below. Figure 1: Melting temperature of DNA is correlated to the % content of G-C bonds in the sequence. The higher the number of GC bonds, the higher the melting temperature. Because even single base pair changes in DNA sequence can influence the T ...

    Get Price
  • Frontiers

    Dictyostelid social amoebae are a highly diverse group of eukaryotic soil microbes that are valuable resources for biological research. Genetic diversity study of these organisms solely relies on molecular phylogenetics of the SSU rDNA gene, which is not ideal for large-scale genetic diversity study. Here, we designed a set of PCR–single-strand conformation polymorphism …

    Get Price
  • Gel Electrophoresis Equipment Selection Guide: Types ...

    1 day agou2002·u2002Three types of gel are used in the electrophoresis process: Agarose gels have low resolution but a high range of separation. They are employed in DNA fragment analysis. Polyacrylamide gels are used for protein and small-fragment DNA analysis. Starch gels are formed from partially hydrolyzed potato starch.

    Get Price
  • 56-81-5 - Glycerol, Cell Culture Grade - 1,2,3 ...

    2021-9-8u2002·u2002In polyacrylamide gel electrophoresis, glycerol is used in sample preparation and gel formation. At a concentration of 5-10%, glycerol is used to increase the density of a sample so that it will layer at the bottom of a sample well. Additional uses include as an aid in casting gradient gels, a protein stabilizer and storage buffer component.

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  • 56-40-6 - Glycine, 0.2M buffer soln., pH 3.5 - Alfa Aesar

    2021-2-4u2002·u2002It is frequently used in the preparation of TG Buffers (Tris-Glycine; sc-296648) where the buffer is used as a running and/or transfer buffer for polyacrylamide gel electrophoresis and western blotting. It is also used as an eluent in column chromatography. Glycine has been reported for use in the enzymatic determination of lactate using lactic ...

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  • 5% SDS 200 mL - SeraCare

    5% SDS is a detergent surfactant commonly used in preparing proteins for sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of proteins and as an additive to transfer buffer in western blotting. SDS increases the elution rate of proteins from the gel. Reference number: 51-20-01. KPL 5% SDS.

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  • Redoxica

    2021-12-9u2002·u2002The Thioredoxin Redox State Assay will determine the relative abundance of oxidized and reduced thioredoxin in cellular lysates, or tissue homogenates. The thiol group is derivatized with an Iodoacetic Acid (IAA) and the oxidized and reduced thioredoxin are separated based on their relative mobility in a native polyacrylamide gel.

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  • Rapid Electronic Autofluorography of Labeled ...

    lipkin, l.e., data-base techniques for multiple two-dimensional polyacrylamide-gel electrophoresis analyses, clinical chemistry 26: 1403 (1980). Google Scholar MAXAM, A.M., NEW METHOD FOR SEQUENCING DNA, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 74 : 560 (1977).

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  • Non-Radioactive Gel Shift Assays - Promega

    The gel shift assay is performed by incubating a purified protein, or a complex mixture of proteins (such as nuclear or whole cell extract preparations), with a labeled DNA fragment containing the putative protein binding site. The reaction products are then analyzed on a nondenaturing polyacrylamide gel. The specificity of the DNA-binding ...

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  • Redoxica

    2021-11-29u2002·u2002Thioredoxin Redox State Assay Kit. Ordering information PRODUCT CODE RE-8011K-40RXN UNIT PRICE $ 598.00 ($14.95 Assays) AVAILABILITY In Stock Ready-to-ship On Nov 29, 2021 FULFILLMENT BY LABSCOOP - SHIPS FROM LITTLE ROCK, AR …

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  • Min Run Gel Electrophoresis System,Hangzhou Bioer ...

    The lid and gel migration board, made in engineering plastics, is designed for intensive use and is etching resist.The electrodes are made of platinum for a long working life. It is suitable for both gelose and polyacrylamide gel. Mini-Run Gel Electrophoresis System Small size, light weight With built-in DC power supply Etching resist and long life

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  • Gel Electrophoresis Equipment: Process and Function ...

    2015-7-7u2002·u2002Gel electrophoresis is useful in forensics, biochemistry, genetics, microbiology and other applications requiring analysis of nucleic acid and protein molecule size and characteristics. Protein electrophoresis is also a common method for analyzing blood plasma in medical applications. Electrophoresis often occurs as a preparatory technique prior to cloning, DNA …

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  • Characterisation of a ω-gliadin gene in Triticum tauschii ...

    2008-1-1u2002·u2002A ω-gliadin gene at the Gli-D t 1 locus of Triticum tauschii accession AUS18913 was isolated using PCR primers, designed from published sequences of ω-gliadin genes of bread wheat cv Cheyenne, and deduced sequences of the N-terminal amino acids of ω-gliadin proteins.Further, the derived protein was isolated from A-PAGE and was sequenced. The …

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  • Antibody-Free Assay for RNA Methyltransferase Activity ...

    For urea denaturing polyacrylamide gel. Thermomixer with SMARTBLOCK 24X 1.5mL TUBES: eppendorf: 5382000023/5361000038: For temperature controlled incubation of 1.5 mL tubes. TOPO TA Cloning Kit: life technologies: Kits for fast cloning of Taq polymerase–amplified PCR products into vectors containing T7 and/or SP6 promoters for in vitro RNA ...

    Get Price
  • how to use 15 cationic polyacrylamide tbe urea gel from ...

    Fractionation of soil humic acids by preparative polyacrylamide gel electrophoresis in the presence of concentrated urea. 2003). Polyacrylamide gel electrophoresis (PAGE) has been used to separate humic substances based on the differences in their molecular size (Castagnola et al. 1979; de Gonzalez et al. 1981).

    Get Price
  • Native Gel Analysis - UNC School of Medicine

    strips migrates into the gel. The leading and trailing ions (acetate/L-alanine) form a boundary that migrates through the gel leaving behind a region of uniform voltage and constant pH (pH 8.8). As this boundary passes the point of sample application (after 10 Vh) the proteins are applied to the gel. The pH in the gel is 8.8 so proteins with ...

    Get Price
  • SDS-PAGE - Assay-Protocol

    2019-7-31u2002·u2002Download SDS-PAGE protocol as a PDF . SDS-PAGE, with full name of sodium dodecyl sulfate polyacrylamide gel electrophores, is the most widely used technique to separate proteins from complicated samples of mixture, plays key roles in molecular biology and wide range of subfield of biological research. Being present a electricity, proteins migerate towards the …

    Get Price
  • Gel electrophoretic separation of proteins from cultured ...

    2014-11-5u2002·u2002Neuroendocrine tumors (NET) often develop asymptomatically and are detected at a late stage. Currently, there exist certain markers of NET that occur only in the advanced stages of the disease. Still, there is need to develop markers specific of the early stage of cancer development. Nevertheless, biomarkers are mostly low‑abundant proteins and require …

    Get Price
  • STR Analysis for Forensic and Paternity Testing

    STR analysis is useful in forensic, paternity and relationship-testing applications. Promega offers STR amplification systems to amplify CODIS loci, ENFSI-recommended loci and other loci such as the low-stutter pentanucleotide markers Penta D and Penta E.

    Get Price
  • Breast Cancer Genetics - Electrophoresis Education kit ...

    2022-1-6u2002·u2002Each kit included enough DNA, agarose gel, electrophoesis buffer and DNA stain for up to 8 groups of students (8 gels). All you need is a horizontal electrophoresis system, power supply and pipettes, all available from Cleaver Scientific. This unit has a 12 month limited warranty, for more information, consult our terms and conditions.

    Get Price
  • Denaturing Gradient Gel Electrophoresis - Cleaver Scientific

    2022-1-10u2002·u2002This technique is called denaturing gradient gel electrophoresis and is explained in more detail below. Figure 1: Melting temperature of DNA is correlated to the % content of G-C bonds in the sequence. The higher the number of GC bonds, the higher the melting temperature. Because even single base pair changes in DNA sequence can influence the T ...

    Get Price
  • Frontiers

    Dictyostelid social amoebae are a highly diverse group of eukaryotic soil microbes that are valuable resources for biological research. Genetic diversity study of these organisms solely relies on molecular phylogenetics of the SSU rDNA gene, which is not ideal for large-scale genetic diversity study. Here, we designed a set of PCR–single-strand conformation polymorphism …

    Get Price
  • Gel Electrophoresis Equipment Selection Guide: Types ...

    1 day agou2002·u2002Three types of gel are used in the electrophoresis process: Agarose gels have low resolution but a high range of separation. They are employed in DNA fragment analysis. Polyacrylamide gels are used for protein and small-fragment DNA analysis. Starch gels are formed from partially hydrolyzed potato starch.

    Get Price
  • (PDF) The use of SDS-PAGE seed protein variability within ...

    The banding patterns produced following polyacrylamide gel electrophoresis of total seed storage proteins in the presence of sodium dodecylsulphate (SDSPAGE) have proved an …

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  • High Protein Binding - Nitrocellulose Membrane

    Prestained proteins were separated in a polyacrylamide gel and electrophoretically transferred to the indicated nitrocellulose membranes. A double layer of membrane was used, one directly against the gel, followed by the second layer. Signal intensity on the second layer is indicative of burn through, which can lead to loss of signal.

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  • Bacterial community and fermentation patterns

    and un wilted Oats in Afghanistan Wali Ajmal Animal Science department, Agriculture Faculty, Sheikh Zayed University, Afghanistan Abstract Aims: To assess the variation in bacterial communities and determine the effects of wilting, storage period and molasses inoculant on the bacterial community and fermentation pattern of oats silage.

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  • Gel Electrophoresis Equipment Selection Guide: Types ...

    1 day agou2002·u2002Three types of gel are used in the electrophoresis process: Agarose gels have low resolution but a high range of separation. They are employed in DNA fragment analysis. Polyacrylamide gels are used for protein and small-fragment DNA analysis. Starch gels are formed from partially hydrolyzed potato starch.

    Get Price
  • 5% SDS 200 mL - SeraCare

    5% SDS is a detergent surfactant commonly used in preparing proteins for sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of proteins and as an additive to transfer buffer in western blotting. SDS increases the elution rate of proteins from the gel. Reference number: 51-20-01. KPL 5% SDS.

    Get Price
  • Identification of Human Decomposed Remains Using the

    2011-3-1u2002·u2002polyacrylamide gel electrophoresis containing 7% glycerol. (In our work, the necessity for denaturing polyacrylamide gel electrophoresis was investigated with respect to resolution and accurate allele identification, and compared resolution for STR alleles was obtained using medium formate vertical gels of both types.)

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  • SKINNET NOURISHING DISCOVERY KIT 2x75ml – Cosmaline

    SKINNET NOURISHING DISCOVERY KIT 2x75ml. Skinnet Nourishing Discovery kit removes dead cells and cleanses your dry skin with an overall nourished glow. It deeply moisturizes your skin, leaving your hands, feet and body soft, supple and beautifully nourished. LBP 38,000.

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  • BROW CRUSH TINTED BROW GEL - SOFT BLACK

    DESCRIPTION INGREDIENTS THINGS ARE SHAPING UP Available in 5 shades Get in the right frame of mind with this lightweight gel that fills, grooms, and shapes those beautiful brows of yours. GRWM: Morphe 2 Brow Gel ft. Lipsticknick SHADE NAMES Blonde Medium Brown Auburn Deep Brown Soft Black Net Weight: 4 mL 0.14 fl. oz. Water (Aqua, Eau), Glyceryl Stearate, …

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  • Native or Denaturing Gel - Which Is For You? - Advansta Inc.

    2015-4-8u2002·u2002Denaturing it. Sample loading buffer for denaturing gels has two key components: The strong detergent, sodium dodecyl sulfate (SDS) and. A reducing agent, either dithiothreitol (DTT) or beta-mercaptoethanol (β-Me). After these are mixed with your sample, you heat it up. The two chemicals plus heat work together to make the charge of the ...

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  • The effects of camel chymosin and Withania coagulans ...

    2021-6-30u2002·u2002Withania coagulans (W. coagulans) extract and camel chymosin have aspartic protease capable of coagulating milk for cheese production. This study investigated the quality of camel and bovine milk ...

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